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KMID : 1149220110190020083
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Korean Journal of Oriental Medical Prescription
2011 Volume.19 No. 2 p.83 ~ p.92
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Monitoring on Microbial flora of Herbal Powder in Long Term Preservation
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Seo Chang-Seob
Shin Hyeun-Kyoo
Shin Kwang-Soo
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Abstract
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Objectives: This study was carried out to moniter microbial flora on freeze-dried herbal powder and identify isolated bacteria.
Methods: We measured the total number of bacteria and fungi in 29 herbal powder which had made according to the guideline of KFDA. For the identification, we observed microscopic properties and carried out polymerase chain reaction(PCR). The purified DNA was analyzed by DNA sequencer.
Results: Among the 29 herbal powders, the fungi were detected only one sample as unacceptable range of total aerobic bacteria. Isolated bacteria were identified as Bacillus cereus, B. subtilis, B. megaterium, B. licheniformis, Erwinia tasmaniensis, E. amylovora, and Pantoea agglomerans by 16S rDNA analysis. E. tasmaniensis was observed 20 herbal samples.
Conclusions: According to above results, further studies for the effective sterilization of low herbal materials should be needed.
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KEYWORD
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monitoring, microbial flora, identification, preservation
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